Related Experiment Video
Updated: May 26, 2026

Oral Health Assessment by Lay Personnel for Older Adults
Published on: February 2, 2020
Which Norm of Mixing Ability Index to Evaluate Deficiency in Masticatory Performance in Young Adults?
Martine Hennequin1, Gaëlle Dol1,2, Thomas Douillard1,2
1CROC, Université Clermont Auvergne, Clermont-Ferrand, France.
Background:
The two-coloured chewing gum test is widely used to quantify oral mixing ability. Lack of consensual normative reference values limits its interpretation and comparability across studies.
Objective:
To present the methodology establishing normative reference cut-offs for masticatory mixing ability in healthy young adults using two-coloured chewing gums.
Methods:
Data from 61 healthy individuals (22.08 ± 2.22 years) who fulfilled anatomical (≥ 7 posterior functional units), psychosocial (GOHAI ≥ 52) and physiological (carrot D50 < 4 mm) criteria for good oral health were considered. Mixing ability was measured after 5 cycles, 20 cycles and 1 min with two different gum types (Hue-Check Gum and Hollywood gum). Outcomes were SD-Hue and Mix%.
Results:
Mix% increased significantly from 5 to 20 cycles for both gums and approached maximal values after 1 min (p < 0.001). At 20 cycles, mean Mix% was 81.06% (95% CI: 78.46-83.66) for Hollywood and 88.31% (95% CI: 85.44-91.17) for Hue-Check Gum. Corresponding mean SD-Hue values were 0.076 (95% CI: 0.068-0.084) and 0.108 (95% CI: 0.085-0.131), respectively. Normative reference cut-offs at 20 cycles were defined using the lower bound of the 95% CI for Mix% (78.46% for Hollywood and 85.44% for Hue-Check Gum) and the upper bound of the 95% CI for SD-Hue (0.084 for Hollywood and 0.131 for Hue-Check Gum).
Conclusion:
Normative reference cut-offs for the two-coloured chewing gum test are provided for healthy young adults, enabling standardized interpretation of mixing outcomes. However, their applicability across other age groups is needed.
Related Concept Videos
Assessment of the Mouth
Mouth Inspection
The inspection begins with visually examining the mouth for symmetry, color, and size.
Mortar Properties
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin and...
