Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Translational Regulation of Sf1 Integrates Alternative Splicing and Hematopoietic Stem Cell Fate.

Blood·2026
Same author

Suppression of AKT Activation Through LZK Inhibition Limits Tumor Growth in Esophageal and Head and Neck Cancers.

Molecular cancer therapeutics·2026
Same author

A dynamic gateway: Uncovering the expanded human TOM complex interactome and its regulatory complexity.

Science advances·2026
Same author

Proteomic profile of Laser-dissected Motoneurons, Ependymal Cell Layer and Dorsal Root Ganglia after Spinal Cord Injury in the Rat.

Scientific data·2026
Same author

MLK4 orchestrates macrophage-induced triple-negative breast cancer invasion and ECM remodeling via enhanced paracrine signaling and NF-κB-MMP axis activation.

Cell death & disease·2026
Same author

Publisher Correction: Polyamine-dependent metabolic shielding regulates alternative splicing.

Nature·2026

Related Experiment Video

Updated: May 26, 2026

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity
09:59

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

Published on: March 31, 2023

Spermidine-Based Activity Probe for ALDH1A1 in Cells.

Tram T B Tran1, Zuzanna Sas1, Kamila Karpińska1

  • 1IMol, Polish Academy of Sciences, Warsaw, Poland.

Chembiochem : a European Journal of Chemical Biology
|May 25, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel chemical probe to study aldehyde dehydrogenase 1A1 (ALDH1A1). This tool reveals that inhibiting ALDH1A1

Keywords:
ALDH1A1activity‐based probealdehyde dehydrogenasebioorthogonal tagspermidine analog

More Related Videos

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
08:17

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Published on: April 16, 2021

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

Related Experiment Videos

Last Updated: May 26, 2026

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity
09:59

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

Published on: March 31, 2023

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
08:17

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Published on: April 16, 2021

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Molecular Biology

Background:

  • Aldehyde dehydrogenase 1A1 (ALDH1A1) is a key enzyme implicated in various cellular processes and cancer.
  • Previous studies often used broad-spectrum inhibitors or high doses, making it difficult to attribute observed effects solely to ALDH1A1 inhibition.
  • Understanding the specific role of ALDH1A1 catalytic activity requires precise tools for target engagement and activity assessment.

Purpose of the Study:

  • To develop and utilize a novel, activity-dependent chemical probe for aldehyde dehydrogenase 1A1 (ALDH1A1).
  • To investigate the consequences of selective ALDH1A1 catalytic activity inhibition in cancer cell lines.
  • To determine if ALDH1A1 catalytic activity loss alone is sufficient to induce previously reported cellular phenotypes.

Main Methods:

  • Synthesis and application of a spermidine analog as a selective ALDH1A1 chemoprobe.
  • Chemoproteomic profiling to confirm ALDH1A1 as the primary cellular target.
  • Activity-dependent labeling and bioactivation of the probe within cells.
  • Treatment of A549 and MDA-MB-468 cancer cell lines with the ALDH1A1 inhibitor NCT-501 at optimized concentrations.
  • Assessment of ALDH1A1 protein abundance, activity, and cellular phenotypes using fluorescence microscopy, in-gel fluorescence, and western blotting.

Main Results:

  • A novel spermidine analog selectively engages and labels ALDH1A1 in cellulo via intracellular bioactivation and irreversible modification.
  • Chemoproteomics confirmed ALDH1A1 as the main target, with the probe labeling within the catalytic/substrate-binding region.
  • Selective inhibition of ALDH1A1 catalytic activity with NCT-501 did not alter protein abundance.
  • Phenotypes previously linked to ALDH1A1 inhibition were not observed under these selective, activity-targeted conditions.

Conclusions:

  • Loss of ALDH1A1 catalytic activity alone is insufficient to elicit the widely attributed cellular responses.
  • Functional redundancy among ALDH isoforms or non-catalytic roles of ALDH1A1 may explain the lack of observed phenotypes.
  • The developed spermidine-based probe offers a valuable tool for activity-aware target engagement and dissecting ALDH1A1 biology in living systems.