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Related Experiment Video

Updated: May 26, 2026

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Published on: October 29, 2016

Methods for evaluating bacterial dispersal on hyphal networks.

Aureline Bouchard¹, Martin Darino², Guillaume Cailleau¹

¹Laboratory of Microbiology, University of Neuchâtel, Neuchâtel CH-2000, Switzerland.

|May 25, 2026

Summary

This summary is machine-generated.

Filamentous fungi create "fungal highways" (FH) for microbial dispersal. New 3D-printed devices enable scalable study of FH formation, revealing nutrient and inoculation impacts on bacterial transport.

Keywords:

3D-printing bacterial transport bacterial-fungal interactions fungal highways mycelial networks

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Investigating Bacterial-Fungal Interactions using Fungal Highway Columns in Diverse Environments and Substrates

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Published on: January 24, 2025

Area of Science:

Microbiology
Mycology
Plant Pathology

Background:

Filamentous fungi and oomycetes utilize hyphae as

Purpose of the Study:

Develop scalable tools for studying hyphal-mediated microbial dispersal.
Investigate factors influencing fungal highway (FH) formation and bacterial transport.

Main Methods:

Designed and utilized 3D-printed devices for simultaneous testing of up to 10 microbial pairs.
Assessed bacterial transport across hyphal networks under varying nutrient conditions and inoculation strategies.
Developed a novel in planta assay for evaluating FH formation during host colonization.

Main Results:

Bacterial transport via FH was inversely correlated with nutrient richness, favoring poorer conditions.
Both cis- and trans-inoculation supported FH formation, with delayed bacterial arrival in non-co-inoculated setups.
FH-mediated bacterial transport was confirmed as relevant for natural substrate colonization and in planta (wheat spike) colonization by Fusarium graminearum.

Conclusions:

3D-printed devices offer accessible, scalable methods for studying hyphal-mediated bacterial dispersal.
Nutrient availability and biological specificity significantly influence FH establishment and function.
FH plays a crucial role in microbial dispersal and host colonization dynamics.