Template specificity of DNA-dependent RNA polymerase from calf liver nuclei

The efficiency of transcription of both mammalian and bacterial templates by 3 distinct multiple forms of DNA-dependent RNA polymerase from calf liver nuclei has been determined. The homologous template and salmon sperm DNA were more efficiently transcribed than bacterial templates. The native DNA/denatured DNA activity ratio of the 2 alpha-amanitin-insensitive forms was found consistent with that described for enzymes exhibiting nucleolar localization.