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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Ribosome Profiling02:24

Ribosome Profiling

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Updated: May 26, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Published on: August 6, 2014

dsRADAR: Imaging and Quantifying Cellular dsRNA by Repurposing RNA Binding Proteins.

Weina Cheng, Tyson D Todd, Harshad Ingle

    Biorxiv : the Preprint Server for Biology
    |May 25, 2026
    PubMed
    Summary

    New dsRNA-binding reagents (dsRADAR) offer improved detection of double-stranded RNA (dsRNA), a molecule implicated in viral infections, immune disorders, and RNA therapeutics. These reagents overcome limitations of existing antibodies, enabling sensitive and reliable dsRNA detection.

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    Last Updated: May 26, 2026

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    Published on: June 12, 2019

    Area of Science:

    • Molecular Biology
    • Immunology
    • Biotechnology

    Background:

    • Double-stranded RNA (dsRNA) triggers innate immunity and is implicated in diseases and RNA therapeutics.
    • Current dsRNA detection methods (J2, K1 antibodies) have limitations in sensitivity and sequence bias.
    • Endogenous dsRNA accumulation can drive harmful immune responses in various conditions.

    Purpose of the Study:

    • To develop reliable, pan-specific affinity reagents for dsRNA detection.
    • To overcome the limitations of existing monoclonal antibodies for dsRNA.

    Main Methods:

    • Systematic screening of human adenosine deaminases acting on RNA (ADARs) dsRNA-binding domains (dsRBDs).
    • Engineering of ADAR3-derived dsRBD constructs (dsRADAR) with varied linker lengths and domain combinations.
    • Validation in cell and tissue models of viral infection and gastric inflammation.

    Main Results:

    • ADAR3 dsRBDs showed reduced sequence dependence, making them suitable for dsRNA detection.
    • Engineered dsRADAR reagents demonstrated superior performance compared to existing dsRNA antibodies.
    • dsRADAR enabled sensitive and reliable imaging and quantification of diverse dsRNA structures.

    Conclusions:

    • dsRADAR reagents provide a sensitive and reliable tool for detecting dsRNA.
    • This advancement has implications for studying dsRNA in viral infections, immune disorders, and RNA therapeutics.
    • The engineered reagents offer improved specificity and sensitivity over current detection methods.