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Related Experiment Video

Updated: May 26, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

BARseq3: a modular system for integrating spatial multi-omics and cellular barcoding in single cells.

Huihui Qi¹, Manjari M-G Anant^2,3,4, Dylan Z Faltine-Gonzalez¹

¹Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.

Biorxiv : the Preprint Server for Biology

|May 25, 2026

Summary

This summary is machine-generated.

BARseq3 integrates cellular barcoding with spatial transcriptomics and translatomics for a comprehensive view of single-cell identity. This modular system offers high efficiency and flexibility for multimodal analysis in tissues.

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Published on: April 7, 2023

Area of Science:

Single-cell biology
Molecular biology
Genomics

Background:

Understanding cellular identity necessitates multimodal measurements at the single-cell level.
Cellular barcoding and spatial omics are key technologies, but integrating them is challenging.
Current methods lack flexibility, efficiency, and support for diverse modalities.

Purpose of the Study:

To develop a modular system integrating cellular barcoding with spatial transcriptomics and translatomics.
To achieve high-efficiency molecular measurements at subcellular resolution within tissues.
To enable a multimodal understanding of cellular identity in various biological contexts.

Main Methods:

Introduction of BARseq3, a novel modular system.
Combination of cellular barcoding with spatial transcriptomics and translatomics.
Application to fixed samples, supporting immunostaining and diverse species.

Main Results:

BARseq3 achieves high-efficiency spatial transcriptomics and translatomics at subcellular resolution.
The system demonstrates compatibility with fixed samples and immunostaining.
BARseq3 is easily extensible to incorporate additional spatial assays.

Conclusions:

BARseq3 provides a flexible and efficient platform for multimodal single-cell analysis.
This technology advances the understanding of cellular identity through integrated molecular profiling.
BARseq3 opens new avenues for spatial multi-omics research in diverse biological samples.