Related Experiment Video
Updated: May 26, 2026
06:18
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Portable Detection of Monkeypox virus Clades using Bridging LAMP
Karen Anderson1,2, Mark Knappenberger1,2, Siril Arockiam1,3
1Biodesign Center for Personalized Diagnostic.
Research Square
|May 25, 2026
Summary
New loop-mediated isothermal amplification (LAMP) assays quickly differentiate monkeypox virus (MPXV) clades. This rapid diagnostic tool aids public health responses to MPXV outbreaks.
Area of Science:
- Virology
- Molecular Biology
- Public Health Diagnostics
Background:
- Monkeypox virus (MPXV) is a zoonotic pathogen causing human outbreaks.
- MPXV has two major clades (I and IIb) with differing clinical severity.
- Accurate clade differentiation is crucial for effective public health strategies during outbreaks.
Purpose of the Study:
- To develop rapid diagnostic assays for MPXV clade differentiation.
- To distinguish between MPXV clade I and clade IIa/IIb.
- To detect broader Orthopoxvirus species.
Main Methods:
- Development of three loop-mediated isothermal amplification (LAMP) assays.
- Exploitation of viral genomic rearrangements and LAMP amplicon length limitations.
- Testing on cell culture lysates and crude lesion specimens from MPXV patients.
Main Results:
- Demonstrated clade specificity between MPXV clade I and clade II.
- Achieved rapid detection of MPXV genomic DNA in under 10 minutes.
- Provided clade-specific results in under an hour using limited instrumentation.
- Successfully adapted a portable microfluidic system for point-of-need testing.
Conclusions:
- Developed sensitive and specific LAMP assays for MPXV clade identification.
- The assays enable rapid, point-of-need diagnostics for MPXV outbreaks.
- This technology supports timely public health interventions for zoonotic orthopoxvirus.
