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Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: May 26, 2026

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Published on: February 16, 2017

MicroRNA networks associated with skeletal aging and WNT pathway modulation.

David Achudhan1,2, David Monroe2,3, Mrunal K Dehankar4,5

  • 1Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Guggenheim 7, 200 First Street SW, Rochester, MN, 55905, USA.

Calcified Tissue International
|May 25, 2026
PubMed
Summary

Cellular senescence drives skeletal aging. This study identified key microRNAs (miRs) regulating bone aging and response to radiation, revealing potential therapeutic targets for skeletal health.

Keywords:
AgingBoneGene regulationSenescencemicroRNA

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Last Updated: May 26, 2026

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Area of Science:

  • * Molecular Biology
  • * Gerontology
  • * Skeletal Biology

Background:

  • * Cellular senescence is a significant factor in skeletal aging, occurring naturally and under conditions like radiotherapy.
  • * Osteocytes, the most abundant bone cell type, play a crucial role in bone health and aging due to their longevity.
  • * MicroRNAs (miRs) are critical regulators of cellular processes, including aging and senescence.

Purpose of the Study:

  • * To identify common differentially regulated microRNAs (miRs) associated with skeletal aging in physiological and radiation-induced contexts.
  • * To investigate the role of specific miRs, including miR-183-5p (WNT pathway) and miR-155-5p (SASP), in skeletal aging.
  • * To explore the therapeutic potential of targeting the WNT pathway, using a Sclerostin antibody (Scl-Ab), in modulating skeletal aging.

Main Methods:

  • * MicroRNA sequencing was performed on mouse femurs and osteocytes from young versus old mice, and on focally radiated versus non-radiated femurs.
  • * Analysis focused on identifying commonly dysregulated miRs across aging and radiation models.
  • * Functional assessments involved using a neutralizing antibody to Sclerostin (Scl-Ab) to investigate WNT pathway and senescence gene regulation, followed by miR sequencing in treated, radiated bones.

Main Results:

  • * miR-135a-5p and miR-671-5p were commonly downregulated in skeletal aging models.
  • * miR-183-5p (WNT pathway regulator) was the sole commonly upregulated miR, while miR-155-5p (SASP regulator) was elevated in two conditions.
  • * Treatment with Scl-Ab downregulated miR-133a-3p, a miR implicated in inhibiting bone metabolism, in radiated bones.

Conclusions:

  • * Specific miRs, including miR-183-5p and miR-155-5p, are key regulators of skeletal aging and senescence.
  • * Modulating the WNT pathway with Scl-Ab impacts miR expression and may counteract accelerated skeletal aging.
  • * The study identifies novel regulatory pathways and potential therapeutic targets for mitigating skeletal aging and radiation-induced bone damage.