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Establishing a Standardized Renal Artery Ligation Procedure for Hypertension in Syrian Golden Hamsters
Xuan Qiu1, Wenlin Zhu2, Yindan Luo1
1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University.
Insights
Researchers refined a surgical method to create a reliable hypertensive hamster model. This model, using renal artery stenosis, aids in studying cardiovascular diseases and hypertension complications.
Area of Science:
- Cardiovascular Research
- Animal Models
- Hypertension Studies
Background:
- Hypertension is a significant global health issue, leading to severe complications like heart failure and kidney disease.
- Syrian golden hamsters naturally express cholesteryl ester transfer protein (CETP) and are prone to diet-induced hyperlipidemia.
- Existing models for studying renal artery stenosis (RAS) and secondary hypertension require refinement for consistency.
Purpose of the Study:
- To develop a standardized and reproducible Syrian golden hamster model for investigating the links between RAS, secondary hypertension, and cardiovascular disease.
- To optimize the surgical procedure for inducing RAS in hamsters using the two-kidney, one-clip (2K1C) Goldblatt model.
- To ensure the model reliably produces sustained hypertension for research purposes.
Main Methods:
- A refined surgical technique for inducing RAS in Syrian golden hamsters through partial renal artery ligation was detailed.
- Critical surgical factors, including isolation technique and ligature material, were emphasized to improve procedural outcomes.
- The two-kidney, one-clip (2K1C) Goldblatt model was adapted and optimized for this specific hamster species.
Main Results:
- The optimized surgical protocol achieved a success rate of approximately 90% in inducing hypertension.
- Treated hamsters consistently exhibited an elevation in systolic blood pressure of 25-30 mmHg.
- The procedure demonstrated high consistency and reproducibility in generating the hypertensive model.
Conclusions:
- The refined renal artery ligation procedure offers a robust method for creating a reproducible hypertensive hamster model.
- This optimized model is highly suitable for research into cardiovascular diseases and the complications of hypertension.
- The standardized protocol enhances the reliability of using Syrian golden hamsters in hypertension and cardiovascular research.
Abstract:
Hypertension is considered a major global health threat, which can lead to severe complications, including vascular remodeling, heart failure, myocardial infarction, and end-stage renal disease. Syrian golden hamsters, a model with naturally expressing cholesteryl ester transfer protein (CETP), are susceptible to diet-induced hyperlipidemia. To establish a reliable and reproducible hamster model for studying the relationship between renal artery stenosis (RAS), secondary hypertension, and cardiovascular disease, the two-kidney, one-clip (2K1C) Goldblatt model was employed. This study details a refined surgical procedure for inducing RAS in Syrian golden hamsters via partial renal artery ligation, emphasizing critical factors such as surgical isolation technique and ligature material selection, which significantly impact outcomes. Implementation of this standardized surgical protocol resulted in approximately 90% of treated hamsters exhibiting a consistent systolic blood pressure elevation of 25-30 mmHg. This optimized renal artery ligation procedure provides a robust method for generating a hypertensive hamster model with high consistency, enhanced reproducibility, and utility for cardiovascular disease research.
