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Related Experiment Video

Updated: May 27, 2026

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
09:33

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Published on: June 19, 2014

A Streamlined Protocol for Single-Molecule Localization Microscopy in Arabidopsis Nuclei.

Salim El-Khoury1, Oleksandr Glushonkov2, Cristel C Carles3

  • 1Institut de Biologie Structurale, CEA, CNRS, Université Grenoble Alpes; Plant and Cell Physiology Lab (LPCV), Bioscience and Biotechnology Institute of Grenoble, Grenoble Alpes University - CNRS - INRAE - CEA.

Journal of Visualized Experiments : Jove
|May 25, 2026
PubMed
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This study introduces a streamlined workflow for super-resolution microscopy in Arabidopsis thaliana nuclei. This method enhances visualization of chromatin organization and epigenetic regulation in plants at the nanoscale.

Area of Science:

  • Plant Biology
  • Cell Biology
  • Microscopy

Background:

  • Confocal microscopy offers limited resolution for plant nuclear chromatin architecture.
  • Super-resolution techniques like Single-Molecule Localization Microscopy (SMLM) are needed for nanoscale visualization.
  • SMLM application in plants is hindered by sample preparation challenges.

Purpose of the Study:

  • To develop a streamlined and reproducible workflow for SMLM imaging of isolated Arabidopsis thaliana nuclei.
  • To overcome technical barriers in plant sample preparation for SMLM.
  • To enable nanoscale investigation of chromatin organization and epigenetic regulation in plants.

Main Methods:

  • Developed a protocol involving seedling fixation, gentle tissue chopping, and centrifugation for nuclei enrichment.

More Related Videos

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
11:25

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

Related Experiment Videos

Last Updated: May 27, 2026

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
09:33

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Published on: June 19, 2014

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
11:25

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

  • Implemented in-liquid fluorophore labeling and immobilization on agarose pads for enhanced stability.
  • Minimized background fluorescence and improved labeling consistency for reproducible imaging.

    • Main Results:

    • Achieved nanoscale resolution for visualizing chromatin domains, histone modifications, and nuclear architecture in Arabidopsis.
    • Demonstrated enhanced clarity and reproducibility in plant nuclear imaging.
    • Successfully adapted SMLM techniques for plant cell biology.

    Conclusions:

    • The presented protocol lowers technical barriers for SMLM in Arabidopsis, facilitating nanoscale studies.
    • Provides a versatile method to investigate plant epigenetic regulation and nuclear topology.
    • Establishes a foundation for applying SMLM to plant systems, bridging the gap with mammalian cell biology.