Related Experiment Video
Updated: May 27, 2026

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
Published on: May 11, 2018
[Capsid Protein of Bacteriophage Beihai32 as a Platform for Construction of Virus-Like Particles Displaying Foreign
A A Zykova1, E S Mardanova1, E A Blokhina1
1Skryabin Institute of Bioengineering, Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences, Moscow, 119071 Russia.
Abstract:
Virus-like particles (VLPs) can be used as nanosized carriers for the presentation of various peptides, including antigens. VLPs formed by self-assembly from capsid proteins of bacteriophages with singlestranded RNA genomes can be obtained in bacterial expression systems. However, to act as a universal platform for antigen presentation, the carrier protein should retain the ability to self-assemble into VLPs upon attachment of long peptides. We have shown that the capsid protein of the bacteriophage Beihai32 can be used as a carrier of peptides at least 238 amino acid residues long. The hybrid capsid protein, to which green fluorescent protein (GFP) was attached at the C-terminus, was expressed in Escherichia coli cells and formed spherical VLPs with a diameter of about 30 nm in vivo. GFP was localized on the surface of these particles and retained the ability to fluoresce. Thus, the bacteriophage Beihai32 capsid protein is an effective platform for constructing VLPs that present long peptide antigens on their surface, and such VLPs can be the basis for new recombinant vaccines.
More Related Videos
08:14Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
Published on: February 14, 2013
10:18Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry
Published on: May 27, 2018
Related Concept Videos
Lytic Cycle of Bacteriophages
DNA Bacteriophages
Viral Structure
Introduction to Virus
Viral Replication: Lytic Cycle
Lysogenic Cycle of Bacteriophages