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C1-2 CSF tap in a 6-month-old infant: illustrative case
Edgar Petrosyan1,2, Arturo Balaguer Townsend2, Vaidehi Mahadev1,2
1Department of Neurological Surgery, Northwestern University Feinberg School of Medicine, Chicago.
Journal of Neurosurgery. Case Lessons
|May 25, 2026
Summary
C1-2 puncture offers a safe alternative for cerebrospinal fluid (CSF) access in infants when lumbar puncture is not possible. This case study details a successful C1-2 puncture in a 6-month-old for meningitis diagnosis.
Area of Science:
- Neurosurgery
- Pediatric Neurology
- Diagnostic Procedures
Background:
- Cerebrospinal fluid (CSF) access is crucial for diagnosing conditions like meningitis.
- Lumbar puncture (LP) is the standard method but may be contraindicated or unfeasible in certain pediatric cases.
- C1-2 puncture presents an alternative route for CSF sampling.
Purpose of the Study:
- To describe the successful performance of a C1-2 puncture in a 6-month-old infant.
- To highlight the technical considerations and safety of this procedure in infancy.
- To evaluate C1-2 puncture as an alternative to LP when contraindicated.
Main Methods:
- A lateral C1-2 puncture was performed under general anesthesia with fluoroscopic guidance.
- Preprocedural imaging and real-time fluoroscopy were used to determine needle trajectory and safety.
- Cerebrospinal fluid was successfully aspirated for laboratory analysis.
Main Results:
- The C1-2 puncture was performed without technical difficulty or procedural complications.
- The infant tolerated the procedure well with no adverse postoperative events.
- This represents a rare instance of C1-2 CSF tap in an infant.
Conclusions:
- C1-2 puncture is a safe and feasible method for CSF sampling in infants when lumbar access is contraindicated or unsuccessful.
- This technique provides a viable option for diagnosing suspected meningitis in infants with specific contraindications.
- Further reports on this rarely utilized approach in infancy are warranted.
Related Concept Videos
Viral Meningitis
Viral meningitis is the most common form of meningitis and is often referred to as aseptic meningitis to indicate the absence of bacterial involvement. It is generally milder than bacterial meningitis, with symptoms including fever, headache, stiff neck, drowsiness, nausea, photophobia, and vomiting. Rarely, more severe manifestations or death may occur. Common causative agents include enteroviruses, particularly coxsackie A and B viruses and echoviruses, all members of the Enterovirus genus...
Cerebrospinal Fluid
Cerebrospinal fluid (CSF) is a colorless liquid that flows around the brain and the spinal cord, playing a vital role in the protection, support, and overall function of the central nervous system (CNS). CSF production, circulation, and absorption are tightly regulated processes essential for the brain and spinal cord to function properly.
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain.
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain.

