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Updated: May 27, 2026

Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
CsWRKY40-CsRAV1 module integrates auxin and brassinosteroid signaling to cooperatively regulate EGCG biosynthesis in
Yanming Tuo1, Xiaofeng Lu1, Qingying Song1
1College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Abstract:
Epigallocatechin-3-gallate (EGCG) is a key secondary metabolite of tea sensory quality and health care function. Auxin and brassinosteroid are important signaling molecules involved in regulating plant secondary metabolism, but the mechanism by which they cooperatively regulate EGCG biosynthesis in tea plants remains unclear. In this study, tea leaves were treated with exogenous indole-3-acetic acid (IAA) and 24-epibrassinolide (BR), and metabolomic together with transcriptomic analyses were used to elucidate how hormone signaling and transcription factors regulate EGCG biosynthesis in tea plants. Compared with the control, combined IAA and BR treatment increased EGCG content in tea plants by 13.25%, indicating a marked enhancement of EGCG accumulation. Additional analysis suggested that CsSAUR and CsBRI1 may be associated with the expression changes of CsWRKY40 and CsRAV1 in response to auxin and brassinosteroid signaling. The results of antisense oligonucleotide (AsODN) inhibition and molecular docking suggested that CsWRKY40 and CsRAV1 may coordinately regulate the expression of the target gene CsPAL in response to auxin and brassinosteroid signals, thereby cooperatively promoting EGCG biosynthesis in tea plants. This study proposes a regulatory framework in which the CsWRKY40-CsRAV1 module may link auxin and brassinosteroid signaling with EGCG biosynthesis in tea plants, providing a new perspective for further investigation of the regulatory mechanisms underlying fresh leaf quality.
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