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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: May 27, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

Multiplexed perturbation enables scalable pooled screens.

Stefan Oberlin^1,2,3, Neil Q Tay^1,2, Albert Xue⁴

¹Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.

|May 25, 2026

Summary

This summary is machine-generated.

Multiplexing multiple guide RNAs in CRISPR screens enhances efficiency and reduces cell number requirements. This strategy maintains screen performance, enabling genome-wide screens with fewer cells, ideal for resource-limited settings.

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Last Updated: May 27, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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Area of Science:

Molecular Biology
Genomics

Background:

CRISPR screens link genes to phenotypes but require large cell numbers.
High cell numbers pose technical and financial challenges for pooled CRISPR screens.

Purpose of the Study:

To investigate co-delivery of multiple guide RNAs (sgRNAs) via high multiplicity of infection (MOI) in CRISPR interference screens.
To enhance screening efficiency and reduce cell number requirements for CRISPR screens.

Main Methods:

Systematic evaluation of screen performance across varying MOIs.
Assessing multiplexing effects on knockdown efficiency, sgRNA representation, and phenotype interference.
Application of optimized conditions to a genome-wide screen for ICAM-1 regulators.

Main Results:

sgRNA multiplexing (MOI 2.5-10) maintains screen performance.
Significant reductions in cell number requirements are achievable with multiplexing.
Successfully identified new ICAM-1 regulators using only half a million cells.

Conclusions:

Multiplexed sgRNA strategies streamline CRISPR screening.
This approach enhances screening efficiency and reduces resource needs.
Provides a framework for resource-limited CRISPR screening applications.