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Updated: May 27, 2026
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Published on: May 24, 2017
An encyclopedic regulatory and functional atlas of RNA interactomes
Keren Zhou1,2,3,4, Junhong Huang5, Shurong Liu5
1MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangdong, China. Keren.Zhou@stjude.org.
Abstract:
RNA molecules interact extensively with each other and with RNA-binding proteins (RBPs) to regulate gene expression. To improve detection of RBP-RNA and RNA-RNA interactions, we developed the rbsSeeker and rriScan tools and integrated the interactions into the Encyclopedia of RNA Interactomes (ENCORI), providing web-based modules to explore RNA interactions. Using this resource, we identified and validated novel N6-methyladenosine (m6A)-associated proteins, an orphan small nucleolar RNA guiding rRNA pseudouridines and target-directed microRNA degradation events, establishing ENCORI as a framework for studying RNA interactomes.
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