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Updated: May 27, 2026

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
Published on: March 11, 2020
Whole-genome sequencing of severe fever with thrombocytopenia syndrome virus using nanopore adaptive sampling
Hye-Ryung Byun1, Seong-Ryeong Ji1, Joon-Seok Chae2
1Laboratory of Veterinary Internal Medicine, BK21 FOUR Future Veterinary Medicine Leading Education and Research Center, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
Abstract:
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne zoonotic pathogen. We evaluated nanopore adaptive sampling (NAS) for whole-genome assembly using two wildlife-derived SFTSV isolates (Vero cell-cultured) sequenced alongside tick-derived samples. Using the PromethION platform, complete L, M, and S segments were successfully assembled in a single run. NAS supported the reconstruction of previously characterized isolate, including an extended S segment, and enabled the characterization of a genotype B-2 isolate. These results suggest that NAS is an effective tool for genomic surveillance of SFTSV under complex sequencing conditions.
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