Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Luminescent Ir<sup>III</sup>-Au<sup>I</sup> Heterobimetallic Complex with a Carbene Bridging Ligand.

Inorganic chemistry·2026
Same author

Glycoconjugated Re(CO)<sub>3</sub><sup>+</sup> complexes: syntheses, characterization, cytotoxicity and cellular localization.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

Integrated online HILIC-ESI-HRMS and ICP-MS/MS for chemical species profiling in transgenic soybean callus exposed to copper nanoparticles.

Analytical and bioanalytical chemistry·2026
Same author

Synthesis, crystal structure and Hirshfeld analysis of the bis-{(<i>E</i>)-2-[1-(benzo[<i>d</i>][1,3]dioxol-5-yl)ethylidene]-<i>N</i>-ethyl-hydrazine-1-carbo-thio-amide-κ<i>S</i>}di-chlorido-mercury(II) complex.

Acta crystallographica. Section E, Crystallographic communications·2026
Same author

Exploring the chemical and biological interplay in phenanthroline-containing copper complexes.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine·2026
Same author

Synthesis, characterization, and biological investigation of thiosemicarbazone-ruthenium(II) complexes.

Journal of inorganic biochemistry·2026
Same journal

Cation-templated synthesis of a Fe<sub>4</sub>Co<sub>20</sub> cyanometallate cluster.

Dalton transactions (Cambridge, England : 2003)·2026
Same journal

High-field multinuclear MAS NMR and synchrotron XANES reveal the influence of strontium salt chemistry on geopolymer nanostructure.

Dalton transactions (Cambridge, England : 2003)·2026
Same journal

Carbonyl insertion into metal-boron based clusters: pathway to a rhodathiacarborane.

Dalton transactions (Cambridge, England : 2003)·2026
Same journal

Simulation of displacement damage in CsPbBr<sub>3</sub> induced by neutron irradiation based on the Monte Carlo method.

Dalton transactions (Cambridge, England : 2003)·2026
Same journal

Photocatalysis-tribocatalysis synergy in oxygen vacancy-rich Zn<sub>2</sub>SnO<sub>4</sub>: mechanism and enhanced all-day performance.

Dalton transactions (Cambridge, England : 2003)·2026
Same journal

Two-dimensional Co/Ni coordination polymers: structure-activity relationship and bifunctional performance for electrocatalysis and energy storage.

Dalton transactions (Cambridge, England : 2003)·2026
See all related articles

Related Experiment Video

Updated: May 27, 2026

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
10:51

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

Published on: April 10, 2015

Ruthenium(II)/diphosphine/naphthoquinone complexes: synthesis, characterization, anticancer activity and molecular

Analu Rocha Costa1,2, Leticia Pires de Oliveira1, Marcos V Palmeira-Mello1

  • 1Departamento de Química, Universidade Federal de São Carlos, CP 676, CEP 13565-905, São Carlos, SP, Brazil. marcos.palmeira@ufscar.br.

Dalton Transactions (Cambridge, England : 2003)
|May 26, 2026
PubMed
Summary
This summary is machine-generated.

New ruthenium complexes show promise as breast cancer treatments. The compound Ru4 demonstrated significant cytotoxicity against triple-negative breast cancer cells, inducing apoptosis and inhibiting colony formation, suggesting a potential new therapeutic strategy.

More Related Videos

Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents
07:20

Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents

Published on: May 28, 2014

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
07:12

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Published on: October 26, 2017

Related Experiment Videos

Last Updated: May 27, 2026

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
10:51

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

Published on: April 10, 2015

Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents
07:20

Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents

Published on: May 28, 2014

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
07:12

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Published on: October 26, 2017

Area of Science:

  • Inorganic Chemistry
  • Medicinal Chemistry
  • Cancer Biology

Background:

  • Triple-negative breast cancer (TNBC) is an aggressive subtype lacking targeted therapies.
  • Current treatment options for TNBC are limited, necessitating novel therapeutic strategies.
  • Ruthenium complexes offer potential as anticancer agents due to their diverse coordination chemistry and biological activities.

Purpose of the Study:

  • To synthesize and characterize novel ruthenium(II) complexes with naphthoquinone ligands for potential breast cancer therapy.
  • To evaluate the in vitro cytotoxicity and mechanism of action of these complexes against breast cancer cell lines.
  • To identify the most promising compound for further development as a breast cancer therapeutic.

Main Methods:

  • Synthesis and characterization of four ruthenium(II) complexes: [Ru(NQ1)(bipy)(dppen)]PF6 (Ru1), [Ru(NQ2)(bipy)(dppen)]PF6 (Ru2), [Ru(NQ1)(bipy)(DPEphos)]PF6 (Ru3), and [Ru(NQ2)(bipy)(DPEphos)]PF6 (Ru4).
  • In vitro cytotoxicity assays using MDA-MB-231, MCF-7 (breast cancer), and A549 (lung cancer) cell lines.
  • Apoptosis induction, cell cycle analysis, Western blot, cell uptake studies, and DNA-binding assays (including molecular docking).

Main Results:

  • All synthesized ruthenium complexes (Ru1-Ru4) exhibited significant in vitro cytotoxicity against tested cancer cell lines, outperforming cisplatin.
  • Ru4 demonstrated the highest selectivity index (SI = 25.5) against the triple-negative breast cancer cell line.
  • Ru4 induced apoptosis, inhibited colony formation, altered cell cycle progression, and showed intracellular uptake in MDA-MB-231 cells.
  • DNA-binding studies confirmed interaction with Ct-DNA via the minor groove.

Conclusions:

  • Ruthenium(II) complexes with naphthoquinone ligands represent a promising class of cytotoxic agents against breast cancer.
  • The compound [Ru(NQ2)(bipy)(DPEphos)]PF6 (Ru4) is identified as a particularly potent and selective agent against triple-negative breast cancer.
  • Ru4's mechanism involves apoptosis induction and DNA interaction, supporting its potential as a novel therapeutic candidate.