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Updated: May 27, 2026

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Rab14 regulates the transport of human papillomavirus to the trans-Golgi network for infectious cell entry

Yoshiyuki Ishii1, Iwao Kukimoto1

  • 1Pathogen Genomics Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan.

FEBS Letters
|May 26, 2026
PubMed

Insights

Human papillomavirus (HPV) uses host protein Rab14 to infect cells. Rab14

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Human papillomavirus (HPV) infection depends on host cell machinery.
  • Rab GTPases are key regulators of intracellular transport.
  • The role of Rab14 in HPV infection is not well understood.

Purpose of the Study:

  • To investigate the role of Rab14 in HPV infection.
  • To determine how Rab14 influences HPV pseudovirion (PsV) entry and intracellular trafficking.

Main Methods:

  • Generated Rab14 knockout (KO) cells.
  • Assessed HPV PsV infection levels in wild-type and KO cells.
  • Utilized confocal microscopy to track PsV localization and colocalization with Rab14.
  • Examined the impact of Rab14 mutants (GDP-bound S25N and GTP-bound Q70L) on infection and localization.

Main Results:

  • Rab14 KO cells showed resistance to HPV PsV infection.
  • PsV transport to the trans-Golgi network (TGN) was significantly reduced in Rab14 KO cells.
  • Reintroduction of wild-type Rab14 restored infection, but mutants did not.
  • Rab14 GTPase cycling influenced PsV colocalization and transport to the TGN.

Conclusions:

  • Rab14 is a critical host factor for HPV infection.
  • Rab14's GTPase cycling regulates HPV transport to the TGN.
  • Targeting Rab14 may offer a strategy to inhibit HPV infection.
Keywords:
Rab14cell entryhuman papillomavirustrans‐Golgi network

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