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Updated: May 28, 2026
08:09
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
Published on: January 7, 2017
Real-time investigation of two conformational changes of an adenosine aptamer with single-molecule fingerprint
ChunMeng Zhong1, JunJie Huang1, Shan Wang1
1College of Chemistry, Sichuan University, Chengdu 610064, P. R. China. zcs@scu.edu.cn.
Chemical Communications (Cambridge, England)
|May 26, 2026
Abstract:
By utilizing fingerprint signals, the conformational changes of an adenosine/aptamer complex were investigated in real time. Two conformations correspond to the kinetic and thermodynamic products, respectively. Furthermore, multi-parameter fingerprint-based discrimination between adenosine and its phosphorylated analogues and an ultrasensitive detection method with a detection limit of 1.75 nM were established.
