Related Experiment Video
Updated: May 28, 2026

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
Published on: May 18, 2020
Enhancing Manufacturing Cell Formation Through Availability-Based Optimization Using the Black Widow Optimizer
Paulo Figueroa-Torrez1, Orlando Duran2, Broderick Crawford3
1Departamento de Ciencias Industriales, Medio Ambiente y Energía, Universidad Católica Boliviana "San Pablo", Colón 734, Tarija, Bolivia.
Abstract:
This study presents a multi-period Generalized Cell Formation Problem with Machine Availability (GCFP-MA) aimed at designing manufacturing cells that explicitly account for equipment reliability, maintainability, and temporal degradation. The proposed model extends classical formulations by introducing (i) availability-based constraints derived from Mean Time Between Failures (MTBF) and Mean Time to Repair (MTTR) and Markov-Chain models, (ii) downtime penalty costs reflecting non-production losses, and (iii) a multi-period horizon that captures system dynamics over time. To solve the resulting NP-hard problem, the Black Widow Optimizer (BWO)-a population-based metaheuristic inspired by cannibalistic reproduction-is implemented and validated against an exhaustive search benchmark. Computational experiments confirm that the BWO attains the global optimum with substantially reduced computational effort, achieving a balanced trade-off between exploration and exploitation. Results highlight that incorporating availability and repair dynamics prevents infeasible or over-optimistic configurations and yields cost-effective, robust cell layouts. The proposed approach provides both theoretical and practical contributions by integrating availability engineering and production system design within a unified optimization framework.
Related Concept Videos
Methods of Medium Optimization
Bioreactor Controls-III
Designing Growth Media for Bioreactors
