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Updated: May 28, 2026
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Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
Published on: March 3, 2023
Detergent-Based Decellularization Preserves Extracellular Matrix Ultrastructure in Ovine Soft Tissues.
Ibrahim E Helal1, Mahmoud F Ahmed2,3, Ahmed M Abdellatif4
1Department of Agriculture, Faculty of Environment Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Biomimetics (Basel, Switzerland)
|May 26, 2026
Summary
This study developed decellularized extracellular matrix (dECM) scaffolds from ovine tissues using a detergent protocol. The resulting dECM scaffolds preserve ECM structure and show potential for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Xenogeneic decellularized extracellular matrix (dECM) scaffolds are promising for tissue engineering.
- Ovine tissues offer a potential source for dECM scaffold development.
Purpose of the Study:
- To develop and characterize dECM scaffolds from ovine skin, tunica vaginalis, fascia lata, and pericardium.
- To evaluate the efficiency of a detergent-based decellularization protocol for ECM preservation.
Main Methods:
- Sequential detergent-based decellularization using sodium dodecyl sulfate and Triton X-100.
- Evaluation through gross examination, histology, SEM, and residual DNA quantification.
Main Results:
- Decellularized tissues showed increased translucency and reduced pigmentation, indicating effective cell removal.
- Histology and SEM confirmed preservation of collagen-rich ECM structure and removal of cellular components.
- Approximately 94% reduction in residual DNA content was achieved across all tissues.
Conclusions:
- The detergent-based protocol reliably produces structurally preserved, acellular scaffolds from multiple ovine tissues.
- These dECM scaffolds possess structural characteristics suitable for potential tissue engineering applications.
