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Angle-Dependent Dip Coating Strategy for Silver Nanostructured Surface Fabrication with Enhanced Fluorescence and
Longchao Qi1, Kaibo Guo1, Xianlong Ning1
1Department of Mechanical Engineering, Yanbian University, Yanji 133002, China.
Biosensors
|May 26, 2026
Summary
Angle-dependent dip coating (ADDC) enables large-area, uniform self-assembly of silver nanoparticles for enhanced fluorescence and Raman scattering. This cost-effective method significantly improves sensitivity for trace detection in biomedical and chemical analysis.
Area of Science:
- Nanotechnology
- Materials Science
- Analytical Chemistry
Background:
- Noble metal nanostructures utilize localized surface plasmon resonance (LSPR) for metal-enhanced fluorescence (MEF) and surface-enhanced Raman scattering (SERS).
- These phenomena significantly enhance sensitivity in trace detection for biomedical and chemical analyses.
- Achieving large-area, uniform, and controllable nanostructures through self-assembly remains a key challenge.
Purpose of the Study:
- To develop an efficient and controllable method for fabricating dual-functional metal-enhanced fluorescence/surface-enhanced Raman scattering (MEF/SERS) substrates.
- To investigate the self-assembly of silver nanoparticles (AgNPs) using angle-dependent dip coating (ADDC) technology.
- To optimize ADDC parameters for large-area uniform nanostructure assembly and evaluate substrate performance.
Main Methods:
- Fabrication of a stable AgNPs-anhydrous ethanol suspension.
- Utilizing angle-dependent dip coating (ADDC) with a microfluidic syringe pump for controlled extraction and deposition.
- Systematic variation of substrate inclination angle and withdrawal flow rate to control nanostructure morphology and assembly.
Main Results:
- ADDC technology enabled efficient, large-area, and uniform self-assembly of silver nanoparticles on glass slides.
- Substrate inclination angle proved more critical for morphology and fluorescence enhancement control than withdrawal flow rate.
- Optimized substrates achieved a Cy3 detection limit of 10⁻¹ nM (MEF) and an R6G SERS detection limit of 10⁻¹⁰ M, with significant enhancement factors.
Conclusions:
- ADDC is a simple, efficient, and cost-effective strategy for producing large-area uniform AgNPs self-assembly.
- The developed substrates exhibit superior dual-function enhancement capabilities for MEF and SERS.
- This method offers a promising approach for highly sensitive trace detection in various analytical applications.
