Development of Multitaxon Indices of Biotic Integrity for Aquatic Ecosystem Health Assessment in Dongjiang Lake

Three locally calibrated Indices of Biotic Integrity (IBIs) based on macroinvertebrates (B-IBI), zooplankton (Z-IBI), and phytoplankton (P-IBI) were developed to characterize relative aquatic ecological condition at impaired sites in Dongjiang Lake, a deep reservoir-type lake in China, during 2021-2023. Using synchronous monitoring data, candidate metrics for the three biotic groups were screened and assembled by integrating taxonomic diversity, community composition, pollution-tolerance attributes, trophic indicators, and functional feeding groups. Metric values were standardized using a linear transformation, and site conditions were classified using a unified five-class grading scheme under the present local calibration framework. A total of 327 taxonomic units (species or morphospecies) were recorded across the three biotic groups, indicating relatively high biodiversity in the study area. Under the present locally calibrated framework, most impaired sites were classified within the moderate-to-good range, with clear interannual variation and spatial heterogeneity. Upstream and downstream sections showed greater fluctuations in IBI classes than the lake area. The macroinvertebrate-based IBI was more sensitive to long-term and cumulative habitat disturbance, whereas the zooplankton- and phytoplankton-based IBIs responded more rapidly to short-term variation in nutrients and water quality. Together, these results indicate that multitaxon IBIs can provide complementary information on relative ecological condition within Dongjiang Lake and may support ecological zoning, pollutant management, and restoration prioritization in similar deep reservoir-type lake systems.