Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Separation, anti-fouling, and chlorine resistance of the polyamide reverse osmosis membrane: From mechanisms to mitigation strategies.

Water research·2021

Same author

Independent effects of seawater pH and high <i>P</i><sub>CO</sub> on olfactory sensitivity in fish: possible role of carbonic anhydrase.

The Journal of experimental biology·2021

Same author

Designing a Single Protein-Chain Reporter for Opioid Detection at Cellular Resolution.

Angewandte Chemie (International ed. in English)·2021

Same author

Matching Ecosystem Services Supply and Demand through Land Use Optimization: A Study of the Guangdong-Hong Kong-Macao Megacity.

International journal of environmental research and public health·2021

Same author

Integration of comprehensive genomic profiling, tumor mutational burden, and PD-L1 expression to identify novel biomarkers of immunotherapy in non-small cell lung cancer.

Cancer medicine·2021

Same author

SARS-CoV-2 nsp12 attenuates type I interferon production by inhibiting IRF3 nuclear translocation.

Cellular & molecular immunology·2021

Search research articles

Related Experiment Video

Updated: May 28, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

CDs/FeCo-ONSs Composite with Peroxidase-like Activity for Ascorbic Acid Detection.

Xue Liu¹, Yuanhang Wei¹, Wenjing Wang¹

¹College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.

Nanomaterials (Basel, Switzerland)

|May 26, 2026

Summary

Nitrogen-doped carbon dots enhance the catalytic activity of iron-cobalt oxide nanosheets. This composite creates a sensitive sensor for detecting ascorbic acid (AA) in food products.

Keywords:

ascorbic acid detection carbon dots iron-cobalt oxide nanosheets nanozyme

More Related Videos

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Published on: June 23, 2020

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

Published on: September 19, 2025

Related Experiment Videos

Last Updated: May 28, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Published on: June 23, 2020

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

Published on: September 19, 2025

Area of Science:

Materials Science
Nanotechnology
Analytical Chemistry

Background:

Iron-cobalt oxide nanosheets (FeCo-ONSs) exhibit intrinsic peroxidase-like (POD-like) activity.
Carbon dots (CDs) are emerging nanomaterials with tunable properties.
Nanozymes offer advantages over traditional enzymes in catalysis and stability.

Purpose of the Study:

To synthesize nitrogen-doped carbon dots (CDs) and combine them with FeCo-ONSs to create a novel nanozyme.
To investigate the enhanced catalytic performance of the composite nanozyme (CDs/FeCo-ONSs).
To develop a sensitive colorimetric sensor for ascorbic acid (AA) detection based on the enhanced nanozyme activity.

Main Methods:

One-pot hydrothermal synthesis of nitrogen-doped CDs using hydroquinone and o-phenylenediamine.
Anchoring CDs onto FeCo-ONSs to form the CDs/FeCo-ONSs composite nanozyme.
Utilizing the enhanced POD-like activity of CDs/FeCo-ONSs for the colorimetric detection of AA via TMB oxidation.

Main Results:

The CDs/FeCo-ONSs composite demonstrated significantly enhanced POD-like activity compared to FeCo-ONSs alone.
A highly sensitive colorimetric sensor for AA was developed with a wide linear range (0.1–50 µM) and a low limit of detection (0.018 µM).
The sensor successfully determined AA levels in commercial beverages and fresh fruits, indicating practical applicability.

Conclusions:

The integration of nitrogen-doped CDs with FeCo-ONSs effectively boosts catalytic performance, creating a superior nanozyme.
The developed CDs/FeCo-ONSs nanozyme enables sensitive and reliable colorimetric detection of ascorbic acid.
This work highlights the potential of CDs/FeCo-ONSs nanozymes for practical applications in food analysis and quality control.