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Transcriptome-Metabolome Integration Deciphers the Metabolic and Transcriptional Reprogramming in Mice Due to Vespa
Jisu Jin1, Guangyuan Jiao1, Xiaolei Huang1
1State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
None:
Venom-mediated systemic toxicity is not fully understood. This study explored the dose-dependent effects of Vespa mandarinia venom (VMV) on mice via integrated transcriptomic and metabolomic analyses. Subcutaneous VMV injection induced dose-dependent hypothermia: 80 μg caused severe transient hypothermia and partial mortality, while 40/60 μg led to reversible hypothermia within 24 h. Whole-blood sequencing identified 2400-3281 differentially expressed genes (DEGs) per group, including 1764 shared DEGs. Immune-related pathways were significantly activated, with hub genes validated by qRT-PCR. Serum metabolomics revealed alterations in organic acids, alkaloids, and other metabolites. Integrative transcriptome-metabolome analysis predicted the potential involvement of various pathways in VMV-induced toxicity, including ferroptosis (shared in low-dose VMV groups) and apoptosis. Cumulatively, this study confirms that VMV induces immunometabolic reprogramming, providing a molecular framework for understanding venom-induced systemic toxicity.
