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Related Experiment Video

Updated: May 28, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

A User-Friendly Protocol for Microinjection into Teleost Embryos to Study Gene Function.

Alicia Mendoza1, Isabella Simon1, Sharmin Hasan2

  • 1Department of Biological Sciences, Sam Houston State University, Huntsville, TX, 77341, USA.

Biochemical Genetics
|May 26, 2026
PubMed
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This study presents a unified, optimized protocol for precise microinjection in zebrafish and medaka embryos. The method ensures high-quality genetic manipulation for developmental biology and disease modeling research.

Area of Science:

  • Developmental Biology
  • Functional Genomics
  • Teleost Model Organisms

Background:

  • Zebrafish and medaka are key teleost models in biological research.
  • High-quality, reproducible microinjections are crucial for genetic studies.
  • Existing protocols lack unification and optimization for both species.

Purpose of the Study:

  • To develop a comprehensive and optimized protocol for microinjection in zebrafish and medaka.
  • To enable efficient, precise, and scalable genetic manipulation in these model organisms.
  • To support advanced functional studies in developmental biology and disease modeling.

Main Methods:

  • Controlled photoperiod breeding tank setup for maximized egg yield and minimized contamination.
  • Detailed procedures for sex identification, pair selection, and enhanced egg collection.
Keywords:
CRISPR-Cas9MedakaMicroinjectionMorpholino knockdownZebrafishmRNA overexpression

More Related Videos

Microinjection of Zebrafish Embryos to Analyze Gene Function
07:18

Microinjection of Zebrafish Embryos to Analyze Gene Function

Published on: March 9, 2009

Microinjection of Medaka Embryos for use as a Model Genetic Organism
07:25

Microinjection of Medaka Embryos for use as a Model Genetic Organism

Published on: December 22, 2010

Related Experiment Videos

Last Updated: May 28, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

Microinjection of Zebrafish Embryos to Analyze Gene Function
07:18

Microinjection of Zebrafish Embryos to Analyze Gene Function

Published on: March 9, 2009

Microinjection of Medaka Embryos for use as a Model Genetic Organism
07:25

Microinjection of Medaka Embryos for use as a Model Genetic Organism

Published on: December 22, 2010

  • Standardized methods for injection gel bed preparation, needle pulling, and pico-liter injector calibration for nanoliter-scale injections.
  • Application for gene knockdown (morpholino), gene knockout (CRISPR-Cas9), and mRNA overexpression.

    • Main Results:

    • Achieved consistent nanoliter-scale injections with minimal variability.
    • Demonstrated successful gene manipulation via morpholino, CRISPR-Cas9, and mRNA overexpression.
    • Observed consistent phenotypic outcomes between morpholino and CRISPR-Cas9 approaches.
    • Reported 97.7% phenotype penetrance in CRISPR-Cas9 knockouts with a triple validation approach.

    Conclusions:

    • The developed protocol is easy, comprehensive, and enables efficient, precise, and scalable genetic manipulation in zebrafish and medaka embryos.
    • This unified approach supports advanced functional studies and disease modeling.
    • The triple validation method confirms gene function across multiple techniques, enhancing reliability.