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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: May 28, 2026

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

Tunable Transcription-Level CRISPR Interference in Vibrio natriegens Using Engineered Single Guide RNAs.

Mincheol Choi1, Gibyuck Byun2, Giho Kim2

  • 1Department of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul08826, Republic of Korea.

ACS Synthetic Biology
|May 26, 2026
PubMed
Summary

Researchers developed a tunable CRISPR interference system for precise gene control in Vibrio natriegens. This advancement enhances its potential as a microbial platform for producing valuable chemicals.

Keywords:
3-hydroxypropionic acidCRISPR interferenceVibrio natriegenslycopenesynthetic sgRNAtunable gene regulation

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Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
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Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

Related Experiment Videos

Last Updated: May 28, 2026

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

Area of Science:

  • Microbiology
  • Synthetic Biology
  • Metabolic Engineering

Background:

  • Vibrio natriegens is a fast-growing bacterium with potential as a microbial production platform.
  • Existing genetic tools for V. natriegens lack precise gene expression control.
  • CRISPR interference (CRISPRi) offers a way to regulate gene expression.

Purpose of the Study:

  • To develop a tunable CRISPR interference system for V. natriegens.
  • To enable multilevel transcriptional regulation for enhanced biological engineering.
  • To improve the production of value-added chemicals using V. natriegens.

Main Methods:

  • Engineered single-guide RNA (sgRNA) variants by modifying tetraloop and flanking regions.
  • Created a synthetic sgRNA library to modulate dCas9 binding affinity.
  • Applied the tunable CRISPRi system for metabolic engineering in V. natriegens.

Main Results:

  • Achieved modular gene repression across multiple targets using engineered sgRNAs.
  • Demonstrated successful redirection of intracellular carbon flux.
  • Increased 3-hydroxypropionic acid production by 2.2-fold and lycopene production by 1.5-fold.

Conclusions:

  • Developed a simple and effective tunable CRISPRi system for V. natriegens.
  • This system allows for precise, multilevel gene regulation.
  • Expanded the utility of V. natriegens as a versatile platform for chemical production.