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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: May 28, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

Uncovering spatially resolved functional genomics with CRISPR screen sequencing.

Haorui Zhang1, Zongxu Zhang2, Peiyu Wang3

  • 1Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100084, China.

Cell
|May 26, 2026
PubMed
Summary

New tools, spatial CRISPR screen sequencing (SPAC-seq) and TARDIS, link gene functions to spatial biology. This research reveals how gene changes impact tumor metastasis and immune responses in spatial omics data.

Keywords:
Cd44Icam1Spp1cancer immunologyin vivo CRISPR screenspatial CRISPR screenspatial genomicsspatial perturbation analysis toolkitspatial sequencing technologyspatial transcriptomics

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Last Updated: May 28, 2026

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Area of Science:

  • Spatial omics
  • Functional genomics
  • Systems biology

Background:

  • Spatial omics advances tissue-level biology understanding.
  • Limited tools exist to link gene perturbations to spatial phenotypes and signaling pathways.

Purpose of the Study:

  • To develop tools for linking gene functional perturbations to spatial phenotypes and signaling pathways.
  • To enable high-throughput spatial functional genomics studies.

Main Methods:

  • Developed spatial CRISPR screen sequencing (SPAC-seq) for high-throughput spatial CRISPR screens.
  • Developed TARDIS (Target Prioritization Toolkit for Perturbation Data in Spatial Omics) for statistical spatial perturbation analysis.
  • Applied SPAC-seq and TARDIS to link gene perturbations to spatial phenotypes and pathways.

Main Results:

  • Uncovered that Icam1 loss in tumor cells promotes metastasis via immune suppression and macrophage polarization.
  • Revealed Cd44's role in regulating CD8+ T cell spatial phenotypes through interaction with Spp1 on macrophages.
  • Demonstrated a model of transcription factor-chemokine receptor axis coupling cell states with chemotaxis.

Conclusions:

  • SPAC-seq and TARDIS provide an effective framework for studying spatially resolved functional genomics.
  • These tools facilitate the investigation of biological and disease contexts using spatial omics.
  • The study advances understanding of gene function in spatial tissue organization and intercellular communication.