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Updated: May 28, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
The synergy of foundation technologies and computer sciences advances microbial expression systems in biomedical
Le Khanh Nguyen1, Diep Thi Hoang2, Stephane Jouannic3
1Faculty of Agricultural Technology, VNU-University of Engineering and Technology, Hanoi, Vietnam.
None:
The convergence of foundational biotechnologies and computational sciences has transformed microbial expression systems in biomedical genetic engineering. Synthetic biology enables precise genetic modifications, while genome editing tools like CRISPR-Cas9 facilitate targeted manipulations with increasing accuracy. Next-generation sequencing and omics technologies provide comprehensive blueprints for optimizing host pathways. Integration with artificial intelligence improves guide RNA design and predictive modeling, enhancing the specificity and efficiency of genome editing. Systems biology and metabolic modeling support rational strain engineering, ensuring robust performance in production environments. Despite these advances, challenges such as off-target effects, metabolic burden, and scale-up limitations remain. This review highlights the synergistic potential of these technologies and proposes strategies to overcome current barriers, advancing the design of microbial systems for therapeutic production.
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