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This summary is machine-generated.

The human plasma proteome is highly stable within individuals over a year, with distinct donor-specific profiles. This stability supports longitudinal biomarker discovery using mass spectrometry.

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Area of Science:

  • Proteomics
  • Biochemistry
  • Immunology

Background:

  • Mass spectrometry advances enable high-throughput plasma proteomics.
  • Understanding plasma proteome stability is crucial for biomarker discovery.

Purpose of the Study:

  • To evaluate intra- and inter-individual variability in the plasma proteome.
  • To assess the stability of proteomic profiles over 12 months in healthy individuals.

Main Methods:

  • Utilized the Tracking Individuals Monthly for Evaluating Stability (TIMES) cohort (51 participants).
  • Analyzed approximately 600 plasma samples across two independent laboratories using mass spectrometry.
  • Employed a support vector machine model for classification accuracy.

Main Results:

  • High correlation of proteomic data between laboratories despite methodological differences.
  • Demonstrated remarkable intra-individual plasma proteome stability over 12 months.
  • Identified significant inter-individual variability, with stable, donor-specific proteomic profiles (98% classification accuracy).
  • Immunoglobulins showed high inter-individual variability but intra-individual stability.
  • C-reactive protein and inflammation markers displayed temporal fluctuations.

Conclusions:

  • Longitudinal sampling is vital for robust biomarker discovery in plasma proteomics.
  • Mass spectrometry-based workflows are robust for analyzing large cohorts.
  • Plasma proteomic profiles are largely stable within individuals, with significant donor-specific signatures, including immunoglobulins.