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Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
Healthcare Associated Infections II: Preventive Measures01:22

Healthcare Associated Infections II: Preventive Measures

Essential infection prevention measures are based on the knowledge of the infection chain, the modes of transmission in healthcare settings, and the use of the best practices in all healthcare settings. Compulsory public reporting of healthcare-associated infection rates is needed to allow individuals and the community to make informed choices regarding selecting a healthcare facility.
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture01:26

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture

Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...

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Related Experiment Video

Updated: May 28, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Monitoring the Burden of Staphylococcus aureus: A Multi-Year Retrospective Study Using Routine Laboratory Data from a

Andrej Minich1, Peter Sabaka2, Vladimír Heger3

  • 1MediXbank, n.o., Novozámocká 1/67, 949 05 Nitra, Slovakia.

Antibiotics (Basel, Switzerland)
|May 27, 2026
PubMed
Summary

Multidrug-resistant Staphylococcus aureus, including MRSA, increased in Slovakia during and after the COVID-19 pandemic, particularly in surgical settings. Methicillin-susceptible S. aureus (MSSA) was more common, driven by colonization rather than infection.

Keywords:
Staphylococcus aureusdata analysismultidrug resistance

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A Tandem Liquid Chromatography&#8211;Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

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Last Updated: May 28, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

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A Tandem Liquid Chromatography&#8211;Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

Area of Science:

  • Epidemiology
  • Infectious Diseases
  • Medical Informatics

Background:

  • Staphylococcus aureus is a major cause of mortality globally, complicated by antimicrobial resistance.
  • Asymptomatic colonization increases infection risk, but data from low- and middle-income countries (LMICs) are scarce.
  • The COVID-19 pandemic impacted S. aureus epidemiology, creating a critical data gap in Slovakia.

Purpose of the Study:

  • To analyze trends in Staphylococcus aureus infections and colonization in Slovakia.
  • To investigate the impact of the COVID-19 pandemic on S. aureus epidemiology.
  • To evaluate the utility of big data analytics in epidemiological research.

Main Methods:

  • Utilized the KNIME Analytics Platform for data integration, preprocessing, and analysis.
  • Analyzed 5 years of routine laboratory diagnostic data from a laboratory information system (LIS).
  • Distinguished between S. aureus colonization and infection based on laboratory data.

Main Results:

  • Multidrug-resistant S. aureus (MDR SA), including MRSA, incidence rose during 2020-2022 and remained elevated post-pandemic.
  • Surgical departments saw MDR MRSA infection rates up to 12.5 per 1000 discharges.
  • Methicillin-susceptible S. aureus (MSSA) incidence was higher overall, primarily due to colonization (up to 71 per 1000 discharges in non-surgical departments).

Conclusions:

  • Big data analytics platforms offer valuable insights for epidemiological research.
  • Differentiating between colonization and infection is crucial for understanding S. aureus epidemiology.
  • Future research should integrate whole-genome sequencing with big data analytics in Slovakia.