Enhanced Recovery of Bioactive Compounds from Rosa canina L. Leaves: A Cascade Approach Using Ultrasounds and...

Area of Science:

• Agricultural Science
• Biotechnology
• Green Chemistry

Background:

• Rosa canina L. leaves are an underutilized agricultural by-product.
• Valorization of agricultural waste is crucial for the circular economy.

Purpose of the Study:

• To develop a cascade strategy for comprehensive valorization of Rosa canina L. leaves.
• To optimize Ultrasound-assisted extraction (UAE) and High-pressure homogenization (HPH) for maximizing bioactive compound yield.

Main Methods:

• Optimized UAE parameters (temperature, time, ethanol concentration) for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity.
• Applied HPH to residual biomass after UAE for secondary extraction of bioactive compounds.
• Compared extraction efficiency from whole vs. ground leaves.

Main Results:

• Optimal UAE conditions (55.5 °C, 69.7 min, 40.8% ethanol) increased TPC yield by 31% and reduced processing time by 22% compared to conventional methods.
• Extraction from whole leaves was more efficient than from ground leaves, potentially eliminating grinding.
• HPH of residual biomass significantly enhanced secondary bioactive compound release, outperforming high-shear mixing.

Conclusions:

• The UAE + HPH cascade process offers a sustainable and energy-efficient method for maximizing antioxidant yield from Rosa canina L. leaves.
• This approach promotes the circular economy by valorizing agricultural by-products.
• The strategy ensures rational resource utilization and increased antioxidant recovery.