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Enzyme-Assisted Phenolic Extract of Murtilla Pomace: A Green Food Additive to Prevent Ozone-Induced Oxidation in
Vicente Gregorio Valenzuela-Bass1,2, Elva Gonzales-Nieto2, Gabriela Valenzuela-Barra1
1Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380492, Chile.
Abstract:
Murtilla pomace (the by-product generated during juice production) shows a high phenolic content. Recovering phenolics from murtilla pomace is a sustainable approach towards zero waste. In this study, murtilla pomace was subjected to enzyme-assisted extraction using Viscozyme. The extracts were analyzed for TPC and phenolic profile. Antioxidant activity was evaluated by chemical-based assays and the antioxidant property was demonstrated for the first time in an ozone-induced oxidation process applied to a raw fish model system. Enzymatic pretreatment with Viscozyme increased the total phenolic content by up to 57%. The antioxidant activity also increased upon enzymatic treatment. The concentration of quercetin was positively affected, while the content of rutin decreased upon enzymatic pretreatment, likely reflecting the enzymatic hydrolysis biotransforming rutin glycoside into quercetin aglycone. Phenolics from murtilla pomace obtained upon enzyme-assisted extraction were shown to be effective as natural antioxidants against ozone-induced oxidation in salmon. Therefore, enzyme-assisted extraction may be an environmentally friendly strategy in the recovery of these natural antioxidants.
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