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CSDE1 Associates with TOM20 and Mitochondrial Protein-Encoding mRNAs in Sensory Neurons
Hoyong Jin1, Eunsu Jang1, Eunhye Park1
1Department of Brain Sciences, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea.
Antioxidants (Basel, Switzerland)
|May 27, 2026
Summary
The RNA-binding protein CSDE1 links mitochondrial protein mRNAs to the import receptor TOM20 in sensory neurons. CSDE1 depletion impacts mitochondrial protein levels and neurite mitochondrial density.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- Neuronal mitochondrial proteostasis is crucial for high metabolic demands.
- This process involves coordinated expression, targeting, and import of nuclear-encoded proteins.
- The role of RNA-binding proteins in this process is not fully understood.
Purpose of the Study:
- To identify RNA-binding proteins associated with mitochondrial protein-encoding mRNAs in sensory neurons.
- To investigate the role of CSDE1 in mitochondrial protein import and neuronal function.
Main Methods:
- Immunoprecipitation followed by sequencing (RIP-Seq) to identify associated mRNAs.
- Immunofluorescence and biochemical fractionation to assess protein and RNA localization.
- Cell culture and knockdown experiments in sensory neurons.
Main Results:
- CSDE1 associates with nuclear-encoded mitochondrial mRNAs, particularly those in inner membrane/matrix and oxidative phosphorylation pathways.
- CSDE1 localizes to mitochondria and interacts with TOM20 in an RNA-independent manner.
- CSDE1 depletion reduces mitochondrial mRNA and protein levels, and decreases mitochondrial puncta density in neurites.
Conclusions:
- CSDE1 is a TOM20-associated RNA-binding protein involved in post-transcriptional regulation of mitochondrial proteins in sensory neurons.
- CSDE1 plays a role in maintaining mitochondrial integrity and function in neuronal processes.
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