Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 28, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

In Vitro Cell Viability and Migration Inhibitory Effects of Isorhamnetin in Non-Small Cell Lung Cancer Cells.

Chengjing Shen1, Taierpuke Maimaiti1, Gulijikere Kuerban1

  • 1College of Pharmacy, Xinjiang Medical University, Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China.

Biomedicines
|May 27, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Variational sparse Gaussian-process method for detecting spatially variable genes and cellular interactions in spatial transcriptomics.

Briefings in bioinformatics·2026
Same author

Tandem repeat polymorphisms are associated with brain structure: results of two large population-based studies.

Genome medicine·2026
Same author

HTZ-1/H2A.Z expression sustains transcriptional programs that regulate Caenorhabditis elegans lifespan.

Mechanisms of ageing and development·2026
Same author

Advancing clinical precision medicine via peripheral blood immune single-cell omics.

Clinical and translational medicine·2026
Same author

3,3'-Di-O-methylellagic Acid Isolated from <i>Euphorbia humifusa</i> Willd Suppresses Prostate Cancer Cell Viability via Regulating VDAC1 Protein Expression.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

STGNET: extending panel coverage in imaging-based spatial transcriptomics using deep generative adversarial networks.

Briefings in bioinformatics·2026

Isorhamnetin (ISO) effectively inhibits non-small cell lung cancer (NSCLC) proliferation and metastasis by inducing apoptosis and cell cycle arrest. This natural flavonoid modulates key genes and proteins, offering potential as a novel NSCLC therapeutic agent.

Area of Science:

  • * Oncology
  • * Pharmacology
  • * Molecular Biology

Background:

  • * Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality globally.
  • * Isorhamnetin (ISO), a dietary flavonoid, shows promise against lung cancer, but its mechanism requires further study.
  • * Understanding ISO's action in NSCLC is crucial for developing new treatments.

Purpose of the Study:

  • * To investigate the anti-cancer effects of Isorhamnetin (ISO) on non-small cell lung cancer (NSCLC) cells.
  • * To elucidate the molecular mechanisms underlying ISO's anti-proliferative and anti-metastatic actions.
  • * To identify key genes and proteins modulated by ISO in NSCLC.

Main Methods:

  • * Assessed cell viability, apoptosis, and cell cycle using MTT assays, Annexin V-FITC/PI staining, and flow cytometry.
Keywords:
apoptosiscell cycleisorhamnetinmolecular mechanismnon-small cell lung cancertranscriptomics

More Related Videos

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

Related Experiment Videos

Last Updated: May 28, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

  • * Evaluated cell migration, invasion, and adhesion via wound healing and Transwell assays.
  • * Employed RNA sequencing (RNA-seq), qRT-PCR, and Western blot to analyze molecular changes.

    • Main Results:

    • * ISO significantly inhibited NSCLC cell viability, induced apoptosis, and caused S-phase arrest.
    • * ISO suppressed NSCLC cell migration, invasion, and adhesion.
    • * Transcriptomic analysis revealed modulation of genes including ELFN1, TMEM186, SETDB1, and FGFBP1 protein.

    Conclusions:

    • * ISO demonstrates potent anti-proliferative and anti-metastatic effects on NSCLC.
    • * Mechanisms involve apoptosis induction, cell cycle arrest, and regulation of a novel gene/protein network.
    • * ISO shows potential as a therapeutic agent for NSCLC, targeting cancer cells and their microenvironment.