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Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

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Welfare Assessment in Equine-Assisted Service (EAS) Horses.

Jéssica Carvalho Seabra¹, Tanja Hess¹, Tessa Finnestead¹

¹Department of Animal Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Animals : an Open Access Journal From MDPI

|May 27, 2026

Summary

This summary is machine-generated.

Equine-Assisted Service horses showed no difference in stress behaviors with different riders. Periodic health checks are crucial for managing potential chronic stress in these therapy animals.

Keywords:

behavior therapy well-being

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Area of Science:

Animal Welfare Science
Equine Behavior
Veterinary Medicine

Background:

Equine-Assisted Service (EAS) horses face stressors impacting welfare.
Previous research focused on stress indicators solely during therapy sessions.

Purpose of the Study:

To evaluate stress and pain behaviors in EAS horses across different rider groups.
To assess horse welfare using behavioral, physiological, and management indicators.

Main Methods:

Observed 10 horses during EAS sessions with 3 rider groups (able-bodied, physically impaired, non-impaired clients).
Collected behavioral data via video analysis and physiological indicators (ACTH, cortisol, WBC) from blood samples.
Conducted time-budget analysis and evaluated housing/management factors.

Main Results:

No significant difference in stress/pain behaviors per second was found between rider groups.
Four horses required medication adjustment due to high baseline ACTH levels.
Reduced lying time and potential chronic stress indicated by immune cell changes and low cortisol.

Conclusions:

Client disability did not directly influence observed stress behaviors in EAS horses.
Regular physiological monitoring is essential for early detection of health issues in working horses.
Further investigation into environmental factors like artificial lighting is needed to optimize horse welfare.