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Dynamic Shifts in Gut Microbiota and Metabolic Pathways of Xinggao Mutton Sheep During Weaning: A Multi-Omics
Xiaolong He1,2, Jingda Yuan1,2, Biao Wang1,2
1Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, No. 22 Zhaojun Road, Hohhot 010031, China.
Abstract:
The composition and function of the intestinal microbiota of Xinggao mutton sheep were explored before and post-weaning using non-target metabolomics. Both 16S rDNA amplicon sequencing technology and serum non-target metabolomics were used to determine changes in sheep metabolites and intestinal flora. A random sampling method was used to collect fecal samples from 20 pre-weaning lambs and 20 lambs post-weaning. Analysis of the intestinal flora identified Firmicutes as the predominant phylum pre-weaning and Bacteroidota as the dominant phylum post-weaning. Joint multi-omics analysis revealed that Oscillospiraceae UCG-005 and Prevotella_9 were significantly correlated with serum L-tyrosine, Indole-3-acrylic acid, and taurine-related metabolites, suggesting their potential roles in modulating digestive health in Xinggao mutton sheep.
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