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Related Experiment Video

Updated: May 28, 2026

Isolation and Identification of Limbal Niche Cells
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Isolation and Identification of Limbal Niche Cells

Published on: October 27, 2023

A Novel Selective Strategy for Bioactive Limbal Stem Cells Primary Culture Using Deep Cryopreservation and IL-1β

Yinglin Liu1, Liling Xu1, Yanmei Li1

  • 1Key Laboratory for Regenerative Medicine, Ministry of Education, Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510630, China.

Cells
|May 27, 2026
PubMed
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Deep cryopreservation combined with IL-1β induction effectively preserves limbal stem cells (LSCs). This strategy enhances LSC stemness and proliferative capacity, improving preparation for limbal stem cell deficiency (LSCD) transplantation.

Area of Science:

  • Ophthalmology
  • Stem Cell Biology
  • Cryobiology

Background:

  • Limbal stem cell (LSC) transplantation is crucial for treating limbal stem cell deficiency (LSCD).
  • Current limitations include poor LSC stemness maintenance during in vitro expansion due to low stem cell proportion and unstable microenvironment.
  • Cryopreserved corneal tissues retain viable LSCs with robust stem cell characteristics.

Purpose of the Study:

  • To develop an effective strategy for preserving and enriching limbal stem cells (LSCs) for transplantation.
  • To investigate the combined effects of deep cryopreservation and IL-1β induction on LSC stemness and function.

Main Methods:

  • Rabbit corneal tissues were deep cryopreserved for extended periods (≥6 months).
  • Limbal tissues were preconditioned using a combination of deep cryopreservation and IL-1β induction.
Keywords:
cryogenic inductioncryopreservationinflammatory factor inductionlimbal stem cellsprimary cell culture

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Last Updated: May 28, 2026

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Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
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Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Published on: October 24, 2018

  • The effects on LSC proportion (p63+), differentiation markers (K3+), colony-forming efficiency, cell cycle phase, and proliferative capacity were assessed.

    • Main Results:

    • The combined strategy maintained p63+ LSC proportions and reduced K3+ differentiated cells from ~80% to 60%.
    • Colony-forming efficiency increased 6.25-fold, with enhanced proliferative capacity and increased G2/M phase cells.
    • The enriched LSC population demonstrated improved stratified epithelial reconstruction potential.

    Conclusions:

    • Deep cryopreservation combined with IL-1β preconditioning is an effective method for processing limbal tissues.
    • This approach successfully preserves and enriches limbal stem cells (LSCs), offering a practical solution for LSC preparation prior to transplantation.
    • Further in vivo studies are warranted to confirm the functional efficacy of these prepared LSCs in ocular surface reconstruction.