In Vitro Effects of Minor Olive Oil Compounds on Prostacyclin/Thromboxane Balance Under Acute High-Glucose Conditions

This study aimed to evaluate the effects of several minor components of extra virgin olive oil (EVOO) on platelet thromboxane and vascular prostacyclin production in rat aortic rings under high-glucose conditions (300 mg/dL), in relation to their potential antioxidant actions. Under hyperglycaemic conditions, thromboxane production was 1.3 times higher, while prostacyclin production was 40.9% lower than in samples with 100 mg/dL glucose in aortic rings, accompanied by marked oxidative stress (65.6% higher than in samples with 100 mg/dL glucose). The compounds tested inhibited thromboxane production in a concentration-dependent manner, with relative potencies (secoiridoid derivatives (IC₅₀ range: 10^-6 M) = triterpenes (10^-6 M) > alcoholic phenols (10^-5 M for hydroxytyrosol and 10^-4 M for the rest)), while preserving prostacyclin production (5-20% inhibition). All compounds also exerted vascular antioxidant effects, reducing oxidative stress markers and enhancing antioxidant parameters (IC₅₀ range: 10^-6-10^-5 M), and these effects were observed under both normoglycaemic (100 mg/dL) and hyperglycaemic (300 mg/dL) conditions.