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Updated: May 28, 2026
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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications
Published on: March 8, 2024
Adult Stem Cell-Derived Intestinal Organoids as In Vitro Models of High-Fat Diet-Related Intestinal Diseases
Yinju Liu1,2, Lanying Chen1,2, Zengcai Liu1,2
1National Pharmaceutical Engineering Center for Solid Preparation of Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.
Biomolecules
|May 27, 2026
Summary
High-fat diets disrupt intestinal lipid metabolism. Fatty acid-treated intestinal organoids (IOs) effectively model these changes, mirroring findings in high-fat diet mice for metabolic disease research.
Area of Science:
- Gastroenterology
- Metabolic Disease Research
- In Vitro Modeling
Background:
- High-fat diets (HFD) dysregulate intestinal lipid metabolism, contributing to metabolic diseases.
- The utility of intestinal organoids (IOs) for studying HFD-induced metabolic changes is not well-established.
Purpose of the Study:
- To evaluate if fatty acid (FA)-treated IOs accurately replicate intestinal lipid metabolism observed in HFD-fed mice.
- To validate IOs as a model for high-fat diet-induced intestinal metabolic alterations.
Main Methods:
- Mice were fed either a normal chow diet (NCD) or HFD for 12 weeks.
- Mouse small intestinal crypt-derived IOs were treated in vitro with an FA cocktail or vehicle.
- Comparative transcriptomic (Omics) analyses were performed on IOs and mouse intestinal tissues.
Main Results:
- HFD mice showed metabolic syndrome phenotypes, including intestinal lipid accumulation and altered gene expression.
- FA-treated IOs exhibited increased budding, lipid droplet accumulation, and pathway changes mirroring HFD effects.
- Omics data revealed strong congruence between FA-treated IOs and HFD mouse intestinal tissues.
Conclusions:
- Fatty acid-treated intestinal organoids successfully recapitulate key morphological and transcriptional features of intestinal lipid metabolism in high-fat diet models.
- IOs serve as a valuable complementary model for investigating intestinal metabolic disorders and the effects of dietary interventions.
