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The Thermal and Histological Evaluation of Lingual Frenulum Incision Using Diode Lasers in an Experimental Model
Adriana Cátia Mazzoni1, Amanda Maria Vieira2, Amanda Rafaelly Honório Mandetta1
1Postgraduation Program in Biophotonic Medicine, Universidade Nove de Julho, São Paulo 01504-000, SP, Brazil.
Journal of Clinical Medicine
|May 27, 2026
Summary
High-power diode lasers (450 nm and 980 nm) can be safely used for lingual frenulum incisions, showing comparable healing to surgical scissors. The 450 nm laser demonstrated faster tissue cooling and better collagen organization post-procedure.
Area of Science:
- Oral surgery
- Laser dentistry
- Tissue engineering
Background:
- Lingual frenectomy/frenotomy often uses thermal instruments for hemostasis and precise cutting.
- The impact of tissue heating from these instruments on biological repair needs further investigation.
Purpose of the Study:
- To compare the thermal and histological effects of 450 nm (blue) and 980 nm (infrared) high-power diode lasers versus surgical scissors for lingual frenulum incisions.
Main Methods:
- An ex vivo study on porcine tongues recorded thermal variations using infrared thermography.
- An in vivo phase utilized instruments selected from the ex vivo phase for comparison.
Main Results:
- Diode lasers, especially 980 nm and 450 nm, caused thermal elevation, with 450 nm showing faster cooling.
- Histological analysis revealed clinically similar tissue repair across groups by day seven.
- The 450 nm laser group exhibited less collagen fiber disorganization on day seven.
Conclusions:
- Both diode lasers are safe for lingual frenulum incisions with appropriate parameters.
- Further clinical research is necessary to define optimal safe and effective laser parameters.
