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Development of a Multi-Channel and Multilayered PDMS Microfluidic Platform for Real-Time Visualization and

Shichao Zhu1, Mieradilijiang Abudupataer1, Zheng Zuo1

  • 1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

Micromachines
|May 27, 2026
PubMed
Summary
This summary is machine-generated.

This study presents a novel microfluidic platform for cell culture, integrating mechanical stimulation and reagent delivery. It enables precise biomechanical studies and real-time observation of cellular responses.

Keywords:
PDMS biopolymercardiovascular modelingmechanical stimulationmechanotransductionmulti-channel microfluidicsmulti-condition parallel testingorgan-on-chip

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Cellular responses to mechanical stimuli are crucial in physiology and disease.
  • Existing platforms often lack integrated control over mechanical forces, reagent delivery, and real-time imaging.
  • Understanding mechanotransduction requires sophisticated tools for controlled experimental conditions.

Purpose of the Study:

  • To develop an integrated microfluidic platform for precise cyclic mechanical stimulation and independent reagent delivery.
  • To enable real-time optical observation of cellular responses to biomechanical cues.
  • To validate the platform's capability for mechanotransduction studies.

Main Methods:

  • Fabrication of a four-layer polydimethylsiloxane (PDMS) microfluidic device.
  • Integration of pneumatic valves for cyclic mechanical stimulation (-20 kPa, 1 Hz, 10% strain).
  • Independent reagent delivery channels and a central cell culture observation area.
  • Finite element analysis (FEA) for strain uniformity assessment.
  • Cell culture of human aortic smooth muscle cells (CRL-1999) and analysis of HIF-1α expression and F-actin alignment.

Main Results:

  • The platform achieved uniform cyclic mechanical stimulation (mean von Mises strain 14.2%, 81.3% uniformity).
  • Cultured cells exhibited significant HIF-1α upregulation (2.5-fold, p<0.01) under cyclic strain.
  • Pronounced F-actin stress fiber alignment was observed, indicating cellular mechanotransduction.
  • Multi-channel capability allowed parallel testing with varied reagent concentrations.

Conclusions:

  • The developed microfluidic platform effectively integrates cyclic mechanical stimulation, reagent delivery, and real-time imaging.
  • The device provides a versatile tool for systematic investigation of cellular mechanotransduction.
  • This technology facilitates advanced research in biomechanics and cell signaling under controlled conditions.