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Weizmannia coagulans Long45 Supplementation Prevents Feline-Derived Shigella flexneri 13-Induced Colitis in Mice by

Xinyu Zhang1, Yuhe Ma1, Haozhen Liu1

  • 1State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

Nutrients
|May 27, 2026
PubMed

Insights

Weizmannia coagulans Long45 supplementation can help alleviate Shigella-induced colitis in mice. This probiotic may protect against intestinal barrier dysfunction by modulating key inflammatory pathways.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Immunology

Background:

  • Shigellosis, caused by Shigella flexneri, is a global health concern, particularly in young children.
  • Feline-derived Shigella flexneri is investigated as a potential pathogen contributing to diarrhea and intestinal barrier dysfunction.
  • The protective role of fecal microbiota from healthy cats against colitis is explored.

Purpose of the Study:

  • To investigate the effects of Weizmannia coagulans Long45 supplementation on Shigella flexneri-induced colitis in mice.
  • To explore the potential mechanisms underlying the protective effects of W. coagulans Long45.
  • To assess the role of feline-derived Shigella flexneri in colitis.

Main Methods:

  • Induction of colitis in mice using feline-derived Shigella flexneri and fecal microbiota.
  • Analysis of inflammatory cytokine mRNA levels (TNF-α, IL-4, IFN-γ, IL-1β, IL-6, IL-18) and apoptosis.
  • 16S rDNA analysis for microbial abundance (Shigella, Bacillus) and bacterial culture for isolating strains.
  • Assessment of colonic barrier function markers (ZO-1, claudin-3, Muc2) and modulation of NF-κB and Nrf2 signaling pathways.

Main Results:

  • Diarrheic cat microbiota worsened colitis, increasing inflammation and apoptosis.
  • Healthy cat microbiota improved body weight, enhanced barrier function (ZO-1, claudin-3, Muc2), and reduced apoptosis.
  • Weizmannia coagulans Long45 significantly alleviated colitis pathology and barrier dysfunction.
  • W. coagulans Long45 modulated NF-κB and Nrf2 signaling pathways.

Conclusions:

  • Feline-derived Shigella flexneri may act as a pathogen causing diarrhea and intestinal barrier issues.
  • Weizmannia coagulans Long45 demonstrates potential as a probiotic for alleviating Shigella-induced colitis.
  • The probiotic effect of W. coagulans Long45 is linked to its ability to interfere with Nrf2 and NF-κB signaling pathways.

Keywords:
ShigellaWeizmannia coagulanscatdiarrhea

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