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Planococcus dechangensis NEAU-ST10-9^T Promotes Maize Seedling Root Development: Evidence from Effective Fluorescence

Qi Zhou^1,2, Zhenyu Huang^1,2, Han Li²

¹Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, National Engineering Laboratory for Industrial Enzymes, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.

|May 27, 2026

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Researchers developed a new method to track beneficial bacteria in soil. This fluorescence labeling technique helps study how *Planococcus dechangensis* interacts with maize roots to promote plant growth.

Area of Science:

Microbiology
Plant Science
Biotechnology

Background:

Understanding plant-rhizosphere microorganism interactions is vital for biofertilizer development.
Tracking microbial behavior in planta requires effective labeling strategies.
Novel bacterial strains often lack established molecular tools for such studies.

Purpose of the Study:

To develop a fluorescence labeling system for the salt-tolerant bacterium *Planococcus dechangensis* NEAU-ST10-9^T.
To investigate the colonization and growth-promoting mechanisms of *P. dechangensis* NEAU-ST10-9^T in maize.

Main Methods:

Engineered a donor strain and shuttle plasmid for conjugation and GFP overexpression in *P. dechangensis* NEAU-ST10-9^T, creating the labeled strain *Pd103*.
Quantified fluorescence intensity of the labeled strain during growth.

Keywords:

P. dechangensis NEAU-ST10-9T conjugation system fluorescence tracking system plant growth-promoting microorganism rhizosphere colonization

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Utilized confocal microscopy to observe *Pd103* colonization in maize roots.

Main Results:

Successfully generated a genetically labeled strain, *Pd103*, with significantly enhanced fluorescence.
Demonstrated that *Pd103* colonized the epidermis and endodermis of maize roots.
*P. dechangensis* NEAU-ST10-9^T significantly increased maize root length by 1.56-fold.

Conclusions:

Established a robust fluorescence labeling and tracking system for *P. dechangensis* NEAU-ST10-9^T.
This system is a valuable tool for studying plant-microbe interactions at cellular and molecular levels.
*P. dechangensis* NEAU-ST10-9^T shows potential as a biofertilizer for maize growth promotion.