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Related Concept Videos

Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the atmosphere, the...
Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme nitrate reductase...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Global Regulatory Systems01:28

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...

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Related Experiment Video

Updated: May 28, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

The NRT1.1-NLP7 Nexus: An Integrative Signaling Nexus from Nitrate Sensing to Systemic Adaptation and

Juanxia Chen1, Ru Chen2, Qian Li2

  • 1National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China.

Plants (Basel, Switzerland)
|May 27, 2026
PubMed
Summary

Plants utilize nitrate as both a nutrient and signal. The integrated Nitrate transporter 1.1 (NRT1.1)-NIN-like protein 7 (NLP7) nexus reveals new mechanisms for reprogramming plant nitrate responses and enhancing nitrogen use efficiency.

Keywords:
NRT1.1-NLP7 nexusnitrate signalingnitrogen use efficiencynuclear retentionrational engineeringtransceptor

More Related Videos

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Related Experiment Videos

Last Updated: May 28, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Nitrate is crucial for plant growth, acting as a nutrient and signaling molecule.
  • Plant nitrate signaling involves transcriptional reprogramming and developmental adjustments.
  • Previously, nitrate sensing and transcriptional responses were viewed as separate pathways.

Purpose of the Study:

  • To review the integrated signaling nexus between the nitrate transceptor NRT1.1 and transcription factor NLP7.
  • To examine the mechanisms coupling nitrate sensing to transcriptional responses.
  • To explore the potential for engineering nitrate use efficiency in crops.

Main Methods:

  • Literature review of recent findings on nitrate signaling.
  • Analysis of coupling mechanisms including Ca2+-dependent phosphorylation and nucleocytoplasmic shuttling.
  • Investigation of MAPK amplification pathways.

Main Results:

  • The NRT1.1-NLP7 nexus integrates plasma membrane nitrate sensing with nuclear responses.
  • Coupling mechanisms involve Ca2+ signaling, protein shuttling, and MAPK pathways.
  • The nexus exhibits conserved and diversified functions across crop species.

Conclusions:

  • The NRT1.1-NLP7 nexus provides a unified model for plant nitrate signaling.
  • A rational design framework can be developed for enhancing nitrogen use efficiency.
  • Bridging structural and synthetic biology enables predictive crop engineering for improved nitrogen utilization.