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  3. Breeding And Molecular Characterization Of Insect-resistant Transgenic Cotton.
  1. Home
  3. Breeding And Molecular Characterization Of Insect-resistant Transgenic Cotton.

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Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton.

Xiaochun Zhang1, Jiangtao Yang1, Yuxiao Chen1

  • 1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

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|May 27, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Insect-resistant transgenic cotton was developed using Agrobacterium-mediated transformation. This new cotton line, BrsC35, offers enhanced pest control, reducing reliance on chemical pesticides for improved crop yields.

Keywords:
insect-resistantmolecular characteristicstransgenic cotton

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Area of Science:

  • Agricultural Science
  • Biotechnology
  • Genetics

Background:

  • Cotton is a vital global cash crop, crucial for agriculture and national economies.
  • Insect pests significantly threaten cotton's growth, yield, and quality, necessitating effective control strategies.
  • Transgenic technology offers a sustainable approach to developing insect-resistant cotton, reducing chemical pesticide use.

Purpose of the Study:

  • To develop insect-resistant transgenic cotton lines with enhanced pest control capabilities.
  • To introduce specific target genes (epsps, cry1Ac, vip3A) into the cotton genome for pest resistance.
  • To characterize the genetic and trait profiles of the resulting transgenic cotton.

Main Methods:

  • Agrobacterium-mediated transformation was employed to introduce the plant expression vector pC2300-VEC into cotton cultivar CCRI 24.
  • Transgenic plants were screened for the presence of target genes and evaluated for insect resistance traits.
  • PacBio sequencing and conventional molecular methods were used for detailed analysis of insertion sites and copy numbers.

    • Main Results:

    • A novel insect-resistant transgenic cotton line, designated BrsC35, was successfully developed.
    • Molecular characterization confirmed the successful integration and expression of the target genes.
    • The study provided comprehensive analysis of the genetic characteristics of the transgenic line.

    Conclusions:

    • The developed transgenic cotton line BrsC35 demonstrates significant potential for insect resistance.
    • This research offers a valuable new germplasm for advancing insect-resistant transgenic cotton cultivation.
    • The findings support the use of genetic engineering to improve cotton's resilience against pests and reduce chemical inputs.