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Related Concept Videos

Responses to Salt Stress02:02

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
Regulation of Transpiration by Stomata02:04

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
Responses to Drought and Flooding02:41

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
Adaptations that Reduce Water Loss01:57

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

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Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
14:43

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Published on: July 23, 2014

ZmPRN1 Negatively Regulates Salt Stress Tolerance by Modulating ROS Homeostasis in Maize (Zea mays L.).

Lei Ma1, Wenzong Li1, Ke Zhang1

  • 1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Plants (Basel, Switzerland)
|May 27, 2026
PubMed
Summary

A newly identified maize gene, ZmPRN1, negatively regulates salt tolerance. This gene

Keywords:
chloroplastmaizereactive oxygen speciessalt stress

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Area of Science:

  • Plant Molecular Biology
  • Abiotic Stress Physiology
  • Maize Genetics

Background:

  • Soil salinization severely limits global maize production.
  • Understanding molecular mechanisms of salt tolerance is crucial for crop improvement.

Purpose of the Study:

  • Identify novel genes involved in maize salt tolerance.
  • Elucidate the function of ZmPRN1 in response to salt stress.

Main Methods:

  • Gene expression analysis under salt and H2O2 stress.
  • Subcellular localization of ZmPRN1.
  • Analysis of Zmprn1 mutant and overexpression lines.
  • Transcriptome sequencing (RNA-Seq).
  • Yeast-two hybrid and split-luciferase assays for protein interactions.

Main Results:

  • ZmPRN1 expression is downregulated by salt stress.
  • ZmPRN1 localizes to the chloroplast.
  • Zmprn1 mutants show enhanced salt tolerance with reduced reactive oxygen species (ROS).
  • ZmPRN1 overexpression lines are salt-sensitive with increased ROS and decreased chlorophyll.
  • ZmPRN1 interacts with chloroplast NDH complex, E3 ligase RING371, and IAA27.

Conclusions:

  • ZmPRN1 acts as a negative regulator of salt tolerance in maize.
  • ZmPRN1 modulates ROS homeostasis, impacting salt stress response.
  • ZmPRN1 is a potential target for breeding salt-tolerant maize varieties.