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Smartphone-Assisted Paper-Based Analytical Device for Rapid Colorimetric Detection of Total Reducing Sugars in Honey
Alicia Carro1, Isela Lavilla1, Carlos Bendicho1
1Centro de Investigación Mariña, Grupo QA2, Edificio CC Experimentais, Departamento de Química Analítica y Alimentaria, Universidade de Vigo, Campus de Vigo, As Lagoas Marcosende, 36310 Vigo, Spain.
Sensors (Basel, Switzerland)
|May 27, 2026
Summary
A new paper-based device (PAD) allows for simple, low-cost colorimetric quantification of reducing sugars in honey using smartphone imaging. This method aids in detecting honey adulteration and ensuring food safety.
Area of Science:
- Analytical Chemistry
- Food Science
- Biochemistry
Background:
- Honey quality and authenticity are critical due to widespread adulteration.
- Reducing sugars (fructose and glucose) are key indicators of honey composition.
- Adulteration practices pose health risks and compromise honey's market value.
Purpose of the Study:
- To develop a rapid, low-cost, and portable method for quantifying reducing sugars in honey.
- To create a paper-based analytical device (PAD) for colorimetric analysis.
- To utilize smartphone technology for image acquisition and data processing.
Main Methods:
- Utilized Benedict's reaction for colorimetric detection of reducing sugars.
- Developed a spot-test paper-based analytical device (PAD).
- Employed smartphone imaging and the Trigit web application for quantitative analysis.
Main Results:
- Established a linear correlation between color intensity and reducing sugar concentration (50-400 mg/L).
- Achieved a limit of detection (LOD) of 11 mg/L and a limit of quantification (LOQ) of 37 mg/L.
- Demonstrated the PAD's effectiveness for rapid honey analysis.
Conclusions:
- The proposed PAD offers a cost-effective, biodegradable, and portable solution for reducing sugar quantification in honey.
- Smartphone-based analysis enables quick data acquisition, making it suitable for field screening.
- This method enhances food safety and quality control, particularly in resource-limited settings.
