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Multi-Component 3D Bioprinted Platform with Sacrificial Matrix and Collagen-Based Bioinks for Skeletal Muscle Tissue
Carmen Mª Granados-Carrera1, Francisco José Calero Castro2,3, Victor M Perez-Puyana4
1Departamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, 41012 Sevilla, Spain.
Polymers
|May 27, 2026
Summary
This study presents a 3D bioprinted platform for skeletal muscle tissue engineering, using polycaprolactone and gelatin with collagen-based bioinks. Extracellular matrix bioinks show promise for creating functional muscle constructs with enhanced cell viability.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Skeletal muscle tissue engineering faces challenges in creating functional, biomimetic constructs.
- Mechanical stimulation and appropriate biomaterials are crucial for engineered muscle development.
Purpose of the Study:
- To develop and evaluate a multi-component 3D bioprinted platform for skeletal muscle tissue engineering.
- To investigate the influence of polycaprolactone architecture, gelatin concentration, and bioink composition on construct properties.
Main Methods:
- Fabrication of a 3D bioprinted platform using polycaprolactone (PCL) support, sacrificial gelatin (GE) matrix, and collagen-based bioinks.
- Systematic evaluation of PCL architecture, GE concentration (0.75-3 wt%), and bioink formulations (collagen, collagen-Matrigel, ECM-based).
- Characterization using rheology, microstructural analysis, and assessment of cell viability and structural organization.
Main Results:
- All bioinks exhibited shear-thinning behavior and suitable viscoelastic properties for bioprinting.
- The PCL/GE platform demonstrated mechanical stability for bioreactor conditions.
- ECM-based bioinks resulted in highly interconnected porous networks, highest cell viability, and improved structural organization.
Conclusions:
- A versatile 3D bioprinting strategy combining mechanical support and biomimetic environments was demonstrated.
- The developed platform shows potential for fabricating functional skeletal muscle constructs.
- Extracellular matrix-based bioinks are promising for advanced skeletal muscle tissue engineering applications.
