Optimization of Polydatin Hydrolysis Process Through Response Surface Methodology for Efficient Resveratrol...

Area of Science:

• Natural Product Chemistry
• Process Optimization
• Biocatalysis

Background:

• Resveratrol, a polyphenol, has broad biological activities and applications in food and pharmaceuticals.
• Production typically involves extraction from natural sources or hydrolysis of polydatin.
• Efficient and optimized methods for resveratrol production are crucial.

Purpose of the Study:

• To refine and optimize the acid-catalyzed hydrolysis conditions for producing resveratrol from polydatin.
• To establish a stable, feasible, and efficient method for enhanced resveratrol yield.

Main Methods:

• Developed a high-performance liquid chromatography (HPLC) method for quantifying polydatin and resveratrol.
• Conducted single-factor experiments to assess temperature, HCl, and ethanol concentration effects.
• Employed response surface methodology (RSM) with a Box-Behnken design for process optimization.

Main Results:

• Hydrolysis kinetics and resveratrol yield were influenced by temperature and HCl concentration.
• Optimal conditions determined via RSM: 70°C, 1.5 M HCl, 85% ethanol, and 5-hour reaction time.
• Validation experiments achieved an average resveratrol yield of 86.01% with high consistency (RSD = 0.56%).

Conclusions:

• The optimized acid-catalytic hydrolysis process using RSM is effective for resveratrol production.
• This method offers a promising and efficient approach for enhancing resveratrol yield from polydatin.
• The process demonstrates stability and feasibility for industrial application.